APOL4 Antibody
APOL4 Antibody
Product Specifications
Product Name Alternative
Apolipoprotein L4, APOL4, apolipoprotein L, 4, APOL-IV, APOLIV, OTTHUMP00000028937, apolipoprotein L-IV
UniProt
Q9BPW4
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-ENLNELTHIHQSLK
Target
APOL4
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 37kDa band observed in Human Placenta lysates (calculated MW of 38.7kDa according to NP_085146.2) . Recommended concentration: 0.3-1μg/mL. Primary incubation was 1 hour. Two additional bands of unknown identity were also consistently observed at 50kDa and at 70kDa. The band at 50kDa was successfully blocked by incubation with the immunising peptide. The other band at 70kDa however was not blocked by the immunizing peptide and it is therefore a non-specific signal.Immunohistochemistry:Paraffin embedded Human Skeletal Muscle. Recommended concentration: 5μg/mL.
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_085146.2, NP_663693.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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