ERN2 Antibody
ERN2 Antibody
Product Specifications
Product Name Alternative
ERN2, endoplasmic reticulum to nucleus signaling 2 , IRE1b, ER to nucleus signalling 2, IRE1 beta, IRE1, S. cerevisiae, homolog of, endoplasmic reticulum to nucleus signalling 2, inositol-requiring 1 beta
UniProt
Q76MJ5
Immunogen
The immunogen for this antibody is: C-QKRLQSPSKQAQPLD
Target
ERN2
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Preliminary experiments gave an approx 37kDa band in Human Duodenum, Pancreas and Placenta lysates after 0.3μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 107kDa according to NP_150296.3. The 37kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Prediction Reactivity
Human
Tested Applications
ELISA
NCBI Accession Number
NP_150296.3
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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