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ERN2 Antibody

ERN2 Antibody

Product Specifications

Product Name Alternative

ERN2, endoplasmic reticulum to nucleus signaling 2 , IRE1b, ER to nucleus signalling 2, IRE1 beta, IRE1, S. cerevisiae, homolog of, endoplasmic reticulum to nucleus signalling 2, inositol-requiring 1 beta

UniProt

Q76MJ5

Immunogen

The immunogen for this antibody is: C-QKRLQSPSKQAQPLD

Target

ERN2

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Preliminary experiments gave an approx 37kDa band in Human Duodenum, Pancreas and Placenta lysates after 0.3μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 107kDa according to NP_150296.3. The 37kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Prediction Reactivity

Human

Tested Applications

ELISA

NCBI Accession Number

NP_150296.3

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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