D130043K22Rik Antibody
D130043K22Rik Antibody
Product Specifications
Product Name Alternative
MKIAA0319, similar to KIAA0319 gene product, 4930451E12Rik, KIAA0319
UniProt
Q5SZV5
Reactivity
Mouse
Immunogen
The immunogen for this antibody is: C-QGKIKQENKPTLH
Target
D130043K22Rik
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Neuroscience
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 150kDa band observed in Mouse Brain lysates (calculated MW of 118kDa according to Mouse NP_001074520.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Velayos-Baeza, Hum Mol Genet. 2008 Mar 15;17 (6) :859-71; PMID: 18063668) . Recommended concentration: 0.5-1.5μg/mL. An additional band of unknown identity was also consistently observed at 25kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Prediction Reactivity
Rat
Tested Applications
ELISA, WB
NCBI Accession Number
NP_001074520.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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