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TRPM8 Antibody

TRPM8 Antibody

Product Specifications

Product Name Alternative

Transient receptor potential cation channel, subfamily M, member 8, LTRPC6, MGC2849, TRPP8, short form of the TRPM8 cationic channel, transient receptor potential subfamily M member 8

UniProt

Q7Z2W7

Immunogen

The immunogen for this antibody is: C-TSEQDKDNWNGQLK

Target

TRPM8

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments gave an approx 28kDa band in lysates of cell line NIH3T3 after 1μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 128kDa according to NP_076985.4. The 28kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Prediction Reactivity

Human, Mouse, Rat

Tested Applications

ELISA

NCBI Accession Number

NP_076985.4

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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