Anti-Bassoon Antibody

Scaffold protein of the presynaptic cytomatrix at the active zone (CAZ) which is the place in the synapse where neurotransmitter is released (PubMed:12812759). After synthesis, participates in the formation of Golgi-derived membranous organelles termed Piccolo-Bassoon transport vesicles (PTVs) that are transported along axons to sites of nascent synaptic contacts (PubMed:19380881). At the presynaptic active zone, regulates the spatial organization of synaptic vesicle cluster, the protein complexes that execute membrane fusion and compensatory endocytosis (By similarity). Functions also in processes other than assembly such as the regulation of specific presynaptic protein ubiquitination by interacting with SIAH1 or the regulation of presynaptic autophagy by associating with ATG5 (By similarity). Mediates also synapse to nucleus communication leading to rUniProtKB:O88778, PubMed:12812759, PubMed:19380881}.

Immunoblotting: use at 1ug/mL. <br><br>Immunohistochemistry: use at 1-10ug/mL. <br><br>Immunofluorescence: use at 1-10ug/mL. <br><br>Positive control: Rat brain tissue lysate <br><br>These are recommended concentrations; <br><br>Enduser should determine optimal concentrations for their applications.

Bassoon, a 420kDa protein, is localized at presynaptic nerve terminals and appears to play a role in the structural and functional organization of the synaptic vesicle cycle. Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains. The C-terminal regions of the polyglutamine domains are of interest since abnormal amplification of this region in some human proteins, such as Huntingtin, can cause late-onset neuro- degeneration.