Anti-Bassoon Antibody

Rabbit Polyclonal Antibody specific to Bassoon

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Zinc finger protein 231

Gene Name

BSN

NCBI Gene ID

UniProt

Q9UPA5

Cellular Locus

Cytoplasm, Cell junction, synapse, presynaptic active zone, Cytoplasm, cytoskeleton, Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein corresponding to aa786-1041 (N-terminal) of human Bassoon (accession no. NM_003458.3).

Target Antigen

Protein bassoon

Target

Bassoon

Clonality

Polyclonal

Type

Antibody

Applications

WB, IHC, IF

Field of Research

Neuroscience

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Scaffold protein of the presynaptic cytomatrix at the active zone (CAZ) which is the place in the synapse where neurotransmitter is released (PubMed:12812759). After synthesis, participates in the formation of Golgi-derived membranous organelles termed Piccolo-Bassoon transport vesicles (PTVs) that are transported along axons to sites of nascent synaptic contacts (PubMed:19380881). At the presynaptic active zone, regulates the spatial organization of synaptic vesicle cluster, the protein complexes that execute membrane fusion and compensatory endocytosis (By similarity). Functions also in processes other than assembly such as the regulation of specific presynaptic protein ubiquitination by interacting with SIAH1 or the regulation of presynaptic autophagy by associating with ATG5 (By similarity). Mediates also synapse to nucleus communication leading to rUniProtKB:O88778, PubMed:12812759, PubMed:19380881}.

Additionnal Information

Immunoblotting: use at 1ug/mL. Immunohistochemistry: use at 1-10ug/mL. Immunofluorescence: use at 1-10ug/mL. Positive control: Rat brain tissue lysate These are recommended concentrations; Enduser should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse, and rat Bassoon. Multiple isoforms are detected.

Formulation

PBS, pH 7.4

Buffer pH

pH 7.4

Target Background

Bassoon, a 420kDa protein, is localized at presynaptic nerve terminals and appears to play a role in the structural and functional organization of the synaptic vesicle cycle. Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains. The C-terminal regions of the polyglutamine domains are of interest since abnormal amplification of this region in some human proteins, such as Huntingtin, can cause late-onset neuro- degeneration.