Multiplex ELISA Kit For Mouse Cytokine - Screen (15-plex)

Product Overview The Multiplex Mouse Cytokine – Screen (15-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 15 biomarkers or analytes. Using just 25 µl of sample per well, up to 80 samples can be assayed for all 15 markers in the panel within 5.5 hours. The Mouse Cytokine – Screen (15-Plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Multiplex plates are built by adsorbing 15 distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results. Assay Ranges Analyte Assay Type Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection GMCSF Sandwich 560 - 0.77 (pg/mL) 392 (pg/mL) 1 (pg/mL) 0.38 (pg/mL) IFNγ Sandwich 516 - 0.71 (pg/mL) 361 (pg/mL) 0.94 (pg/mL) 0.35 (pg/mL) IL-1α Sandwich 610 - 0.84 (pg/mL) 427 (pg/mL) 1.1 (pg/mL) 0.42 (pg/mL) IL-1β Sandwich 2500 - 3.43 (pg/mL) 1750 (pg/mL) 4.5 (pg/mL) 1.71 (pg/mL) IL-2 Sandwich 360 - 0.49 (pg/mL) 252 (pg/mL) 0.6 (pg/mL) 0.25 (pg/mL) IL-3 Sandwich 60 - 0.08 (pg/mL) 42 (pg/mL) 0.1 (pg/mL) 0.04 (pg/mL) IL-4 Sandwich 400 - 0.55 (pg/mL) 280 (pg/mL) 0.7 (pg/mL) 0.27 (pg/mL) IL-5 Sandwich 546 - 0.75 (pg/mL) 382 (pg/mL) 0.96 (pg/mL) 0.37 (pg/mL) IL-6 Sandwich 504 - 0.69 (pg/mL) 353 (pg/mL) 0.91 (pg/mL) 0.35 (pg/mL) IL-10 Sandwich 667 - 0.91 (pg/mL) 467 (pg/mL) 1.33 (pg/mL) 0.46 (pg/mL) IL-12p70 Sandwich 572 - 0.78 (pg/mL) 400 (pg/mL) 1.01 (pg/mL) 0.39 (pg/mL) IL-17A Sandwich 760 - 1.04 (pg/mL) 532 (pg/mL) 1.4 (pg/mL) 0.52 (pg/mL) MIP-1α Sandwich 1000 - 1.37 (pg/mL) 700 (pg/mL) 1.8 (pg/mL) 0.69 (pg/mL) RANTES Sandwich 1653 - 2.27 (pg/mL) 1157 (pg/mL) 2.92 (pg/mL) 1.13 (pg/mL) TNFα Sandwich 429 - 0.59 (pg/mL) 300 (pg/mL) 0.69 (pg/mL) 0.29 (pg/mL) Analyte Assay Type Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection Analyte Assay Type Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection Analyte Assay Type Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection GMCSF Sandwich 560 - 0.77 (pg/mL) 392 (pg/mL) 1 (pg/mL) 0.38 (pg/mL) IFNγ Sandwich 516 - 0.71 (pg/mL) 361 (pg/mL) 0.94 (pg/mL) 0.35 (pg/mL) IL-1α Sandwich 610 - 0.84 (pg/mL) 427 (pg/mL) 1.1 (pg/mL) 0.42 (pg/mL) IL-1β Sandwich 2500 - 3.43 (pg/mL) 1750 (pg/mL) 4.5 (pg/mL) 1.71 (pg/mL) IL-2 Sandwich 360 - 0.49 (pg/mL) 252 (pg/mL) 0.6 (pg/mL) 0.25 (pg/mL) IL-3 Sandwich 60 - 0.08 (pg/mL) 42 (pg/mL) 0.1 (pg/mL) 0.04 (pg/mL) IL-4 Sandwich 400 - 0.55 (pg/mL) 280 (pg/mL) 0.7 (pg/mL) 0.27 (pg/mL) IL-5 Sandwich 546 - 0.75 (pg/mL) 382 (pg/mL) 0.96 (pg/mL) 0.37 (pg/mL) IL-6 Sandwich 504 - 0.69 (pg/mL) 353 (pg/mL) 0.91 (pg/mL) 0.35 (pg/mL) IL-10 Sandwich 667 - 0.91 (pg/mL) 467 (pg/mL) 1.33 (pg/mL) 0.46 (pg/mL) IL-12p70 Sandwich 572 - 0.78 (pg/mL) 400 (pg/mL) 1.01 (pg/mL) 0.39 (pg/mL) IL-17A Sandwich 760 - 1.04 (pg/mL) 532 (pg/mL) 1.4 (pg/mL) 0.52 (pg/mL) MIP-1α Sandwich 1000 - 1.37 (pg/mL) 700 (pg/mL) 1.8 (pg/mL) 0.69 (pg/mL) RANTES Sandwich 1653 - 2.27 (pg/mL) 1157 (pg/mL) 2.92 (pg/mL) 1.13 (pg/mL) TNFα Sandwich 429 - 0.59 (pg/mL) 300 (pg/mL) 0.69 (pg/mL) 0.29 (pg/mL) GMCSF Sandwich 560 - 0.77 (pg/mL) 392 (pg/mL) 1 (pg/mL) 0.38 (pg/mL) GMCSF Sandwich 560 - 0.77 (pg/mL) 392 (pg/mL) 1 (pg/mL) 0.38 (pg/mL) IFNγ Sandwich 516 - 0.71 (pg/mL) 361 (pg/mL) 0.94 (pg/mL) 0.35 (pg/mL) IFNγ Sandwich 516 - 0.71 (pg/mL) 361 (pg/mL) 0.94 (pg/mL) 0.35 (pg/mL) IL-1α Sandwich 610 - 0.84 (pg/mL) 427 (pg/mL) 1.1 (pg/mL) 0.42 (pg/mL) IL-1α Sandwich 610 - 0.84 (pg/mL) 427 (pg/mL) 1.1 (pg/mL) 0.42 (pg/mL) IL-1β Sandwich 2500 - 3.43 (pg/mL) 1750 (pg/mL) 4.5 (pg/mL) 1.71 (pg/mL) IL-1β Sandwich 2500 - 3.43 (pg/mL) 1750 (pg/mL) 4.5 (pg/mL) 1.71 (pg/mL) IL-2 Sandwich 360 - 0.49 (pg/mL) 252 (pg/mL) 0.6 (pg/mL) 0.25 (pg/mL) IL-2 Sandwich 360 - 0.49 (pg/mL) 252 (pg/mL) 0.6 (pg/mL) 0.25 (pg/mL) IL-3 Sandwich 60 - 0.08 (pg/mL) 42 (pg/mL) 0.1 (pg/mL) 0.04 (pg/mL) IL-3 Sandwich 60 - 0.08 (pg/mL) 42 (pg/mL) 0.1 (pg/mL) 0.04 (pg/mL) IL-4 Sandwich 400 - 0.55 (pg/mL) 280 (pg/mL) 0.7 (pg/mL) 0.27 (pg/mL) IL-4 Sandwich 400 - 0.55 (pg/mL) 280 (pg/mL) 0.7 (pg/mL) 0.27 (pg/mL) IL-5 Sandwich 546 - 0.75 (pg/mL) 382 (pg/mL) 0.96 (pg/mL) 0.37 (pg/mL) IL-5 Sandwich 546 - 0.75 (pg/mL) 382 (pg/mL) 0.96 (pg/mL) 0.37 (pg/mL) IL-6 Sandwich 504 - 0.69 (pg/mL) 353 (pg/mL) 0.91 (pg/mL) 0.35 (pg/mL) IL-6 Sandwich 504 - 0.69 (pg/mL) 353 (pg/mL) 0.91 (pg/mL) 0.35 (pg/mL) IL-10 Sandwich 667 - 0.91 (pg/mL) 467 (pg/mL) 1.33 (pg/mL) 0.46 (pg/mL) IL-10 Sandwich 667 - 0.91 (pg/mL) 467 (pg/mL) 1.33 (pg/mL) 0.46 (pg/mL) IL-12p70 Sandwich 572 - 0.78 (pg/mL) 400 (pg/mL) 1.01 (pg/mL) 0.39 (pg/mL) IL-12p70 Sandwich 572 - 0.78 (pg/mL) 400 (pg/mL) 1.01 (pg/mL) 0.39 (pg/mL) IL-17A Sandwich 760 - 1.04 (pg/mL) 532 (pg/mL) 1.4 (pg/mL) 0.52 (pg/mL) IL-17A Sandwich 760 - 1.04 (pg/mL) 532 (pg/mL) 1.4 (pg/mL) 0.52 (pg/mL) MIP-1α Sandwich 1000 - 1.37 (pg/mL) 700 (pg/mL) 1.8 (pg/mL) 0.69 (pg/mL) MIP-1α Sandwich 1000 - 1.37 (pg/mL) 700 (pg/mL) 1.8 (pg/mL) 0.69 (pg/mL) RANTES Sandwich 1653 - 2.27 (pg/mL) 1157 (pg/mL) 2.92 (pg/mL) 1.13 (pg/mL) RANTES Sandwich 1653 - 2.27 (pg/mL) 1157 (pg/mL) 2.92 (pg/mL) 1.13 (pg/mL) TNFα Sandwich 429 - 0.59 (pg/mL) 300 (pg/mL) 0.69 (pg/mL) 0.29 (pg/mL) TNFα Sandwich 429 - 0.59 (pg/mL) 300 (pg/mL) 0.69 (pg/mL) 0.29 (pg/mL) Kit Components Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip well format, please contact us for customization. Reagents Include: Calibrator: Lyophilized, recombinant antigens in a buffered protein base Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives Streptavidin HRP (1X) : Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase Wash Buffer (20X) : Liquid, 50 mL/vial of a concentrated solution of buffered surfactant Plate Seals (3) : Adhesive strips