Multiplex ELISA Kit For Human Cytokine - Screen (16-plex)

Product Overview The Multiplex Human Cytokine – Screen (16-plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 16 biomarkers or analytes. Multiple analyte measurements are achieved by adsorbing 16 distinct capture antibodies in a defined array to the bottom of each well of a 96-well plate. Our high-quality reagents help ensure the accuracy and precision of your results. Using only 25 µl of sample per well, up to 80 samples can be assayed for all 16 markers in the panel within 2.25 hours. The Multiplex Human Cytokine – Screen (16-plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Assay Ranges Analyte Assay Type Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection IFNγ Sandwich 8000 -10.97 (pg/mL) 5600 (pg/mL) 14.3 (pg/mL) 4.95 (pg/mL) IL-1α Sandwich 4000 -5.49 (pg/mL) 2800 (pg/mL) 7.1 (pg/mL) 5.43 (pg/mL) IL-1β Sandwich 12226 -16.77 (pg/mL) 8558 (pg/mL) 21.8 (pg/mL) 12.40 (pg/mL) IL-2 Sandwich 5213 – 7.15 (pg/mL) 3649 (pg/mL) 9.3 (pg/mL) 4.12 (pg/mL) IL-4 Sandwich 1676 – 2.30 (pg/mL) 1173 (pg/mL) 3.0 (pg/mL) 1.79 (pg/mL) IL-5 Sandwich 3000 – 4.12 (pg/mL) 2100 (pg/mL) 5.3 (pg/mL) 2.90 (pg/mL) IL-6 Sandwich 3000 – 4.12 (pg/mL) 2100 (pg/mL) 5.3 (pg/mL) 3.47 (pg/mL) IL-8 Sandwich 2000 – 2.74 (pg/mL) 1400 (pg/mL) 3.6 (pg/mL) 1.37 (pg/mL) IL-10 Sandwich 8039 – 11.03 (pg/mL) 5627 (pg/mL) 14.3 (pg/mL) 10.99 (pg/mL) IL-12p70 Sandwich 3000 – 4.12 (pg/mL) 2100 (pg/mL) 5.3 (pg/mL) 3.20 (pg/mL) IL-13 Sandwich 3000 – 4.12 (pg/mL) 2100 (pg/mL) 5.3 (pg/mL) 4.12 (pg/mL) IL-15 Sandwich 10733 – 14.72 (pg/mL) 7513 (pg/mL) 19.1 (pg/mL) 6.87 (pg/mL) IL-17A Sandwich 16772 – 23.01 (pg/mL) 11740 (pg/mL) 29.9 (pg/mL) 22.57 (pg/mL) IL-23 Sandwich 41700 – 57.20 (pg/mL) 21000 (pg/mL) 120 (pg/mL) 48.65 (pg/mL) TNFα Sandwich 6500 – 8.92 (pg/mL) 4522 (pg/mL) 11.5 (pg/mL) 7.74 (pg/mL) TNFβ Sandwich 4000 – 5.49 (pg/mL) 2800 (pg/mL) 7.1 (pg/mL) 5.29 (pg/mL) Analyte Assay Type Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection Analyte Assay Type Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection Analyte Assay Type Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection IFNγ Sandwich 8000 -10.97 (pg/mL) 5600 (pg/mL) 14.3 (pg/mL) 4.95 (pg/mL) IL-1α Sandwich 4000 -5.49 (pg/mL) 2800 (pg/mL) 7.1 (pg/mL) 5.43 (pg/mL) IL-1β Sandwich 12226 -16.77 (pg/mL) 8558 (pg/mL) 21.8 (pg/mL) 12.40 (pg/mL) IL-2 Sandwich 5213 – 7.15 (pg/mL) 3649 (pg/mL) 9.3 (pg/mL) 4.12 (pg/mL) IL-4 Sandwich 1676 – 2.30 (pg/mL) 1173 (pg/mL) 3.0 (pg/mL) 1.79 (pg/mL) IL-5 Sandwich 3000 – 4.12 (pg/mL) 2100 (pg/mL) 5.3 (pg/mL) 2.90 (pg/mL) IL-6 Sandwich 3000 – 4.12 (pg/mL) 2100 (pg/mL) 5.3 (pg/mL) 3.47 (pg/mL) IL-8 Sandwich 2000 – 2.74 (pg/mL) 1400 (pg/mL) 3.6 (pg/mL) 1.37 (pg/mL) IL-10 Sandwich 8039 – 11.03 (pg/mL) 5627 (pg/mL) 14.3 (pg/mL) 10.99 (pg/mL) IL-12p70 Sandwich 3000 – 4.12 (pg/mL) 2100 (pg/mL) 5.3 (pg/mL) 3.20 (pg/mL) IL-13 Sandwich 3000 – 4.12 (pg/mL) 2100 (pg/mL) 5.3 (pg/mL) 4.12 (pg/mL) IL-15 Sandwich 10733 – 14.72 (pg/mL) 7513 (pg/mL) 19.1 (pg/mL) 6.87 (pg/mL) IL-17A Sandwich 16772 – 23.01 (pg/mL) 11740 (pg/mL) 29.9 (pg/mL) 22.57 (pg/mL) IL-23 Sandwich 41700 – 57.20 (pg/mL) 21000 (pg/mL) 120 (pg/mL) 48.65 (pg/mL) TNFα Sandwich 6500 – 8.92 (pg/mL) 4522 (pg/mL) 11.5 (pg/mL) 7.74 (pg/mL) TNFβ Sandwich 4000 – 5.49 (pg/mL) 2800 (pg/mL) 7.1 (pg/mL) 5.29 (pg/mL) IFNγ Sandwich 8000 -10.97 (pg/mL) 5600 (pg/mL) 14.3 (pg/mL) 4.95 (pg/mL) IFNγ Sandwich 8000 -10.97 (pg/mL) 5600 (pg/mL) 14.3 (pg/mL) 4.95 (pg/mL) IL-1α Sandwich 4000 -5.49 (pg/mL) 2800 (pg/mL) 7.1 (pg/mL) 5.43 (pg/mL) IL-1α Sandwich 4000 -5.49 (pg/mL) 2800 (pg/mL) 7.1 (pg/mL) 5.43 (pg/mL) IL-1β Sandwich 12226 -16.77 (pg/mL) 8558 (pg/mL) 21.8 (pg/mL) 12.40 (pg/mL) IL-1β Sandwich 12226 -16.77 (pg/mL) 8558 (pg/mL) 21.8 (pg/mL) 12.40 (pg/mL) IL-2 Sandwich 5213 – 7.15 (pg/mL) 3649 (pg/mL) 9.3 (pg/mL) 4.12 (pg/mL) IL-2 Sandwich 5213 – 7.15 (pg/mL) 3649 (pg/mL) 9.3 (pg/mL) 4.12 (pg/mL) IL-4 Sandwich 1676 – 2.30 (pg/mL) 1173 (pg/mL) 3.0 (pg/mL) 1.79 (pg/mL) IL-4 Sandwich 1676 – 2.30 (pg/mL) 1173 (pg/mL) 3.0 (pg/mL) 1.79 (pg/mL) IL-5 Sandwich 3000 – 4.12 (pg/mL) 2100 (pg/mL) 5.3 (pg/mL) 2.90 (pg/mL) IL-5 Sandwich 3000 – 4.12 (pg/mL) 2100 (pg/mL) 5.3 (pg/mL) 2.90 (pg/mL) IL-6 Sandwich 3000 – 4.12 (pg/mL) 2100 (pg/mL) 5.3 (pg/mL) 3.47 (pg/mL) IL-6 Sandwich 3000 – 4.12 (pg/mL) 2100 (pg/mL) 5.3 (pg/mL) 3.47 (pg/mL) IL-8 Sandwich 2000 – 2.74 (pg/mL) 1400 (pg/mL) 3.6 (pg/mL) 1.37 (pg/mL) IL-8 Sandwich 2000 – 2.74 (pg/mL) 1400 (pg/mL) 3.6 (pg/mL) 1.37 (pg/mL) IL-10 Sandwich 8039 – 11.03 (pg/mL) 5627 (pg/mL) 14.3 (pg/mL) 10.99 (pg/mL) IL-10 Sandwich 8039 – 11.03 (pg/mL) 5627 (pg/mL) 14.3 (pg/mL) 10.99 (pg/mL) IL-12p70 Sandwich 3000 – 4.12 (pg/mL) 2100 (pg/mL) 5.3 (pg/mL) 3.20 (pg/mL) IL-12p70 Sandwich 3000 – 4.12 (pg/mL) 2100 (pg/mL) 5.3 (pg/mL) 3.20 (pg/mL) IL-13 Sandwich 3000 – 4.12 (pg/mL) 2100 (pg/mL) 5.3 (pg/mL) 4.12 (pg/mL) IL-13 Sandwich 3000 – 4.12 (pg/mL) 2100 (pg/mL) 5.3 (pg/mL) 4.12 (pg/mL) IL-15 Sandwich 10733 – 14.72 (pg/mL) 7513 (pg/mL) 19.1 (pg/mL) 6.87 (pg/mL) IL-15 Sandwich 10733 – 14.72 (pg/mL) 7513 (pg/mL) 19.1 (pg/mL) 6.87 (pg/mL) IL-17A Sandwich 16772 – 23.01 (pg/mL) 11740 (pg/mL) 29.9 (pg/mL) 22.57 (pg/mL) IL-17A Sandwich 16772 – 23.01 (pg/mL) 11740 (pg/mL) 29.9 (pg/mL) 22.57 (pg/mL) IL-23 Sandwich 41700 – 57.20 (pg/mL) 21000 (pg/mL) 120 (pg/mL) 48.65 (pg/mL) IL-23 Sandwich 41700 – 57.20 (pg/mL) 21000 (pg/mL) 120 (pg/mL) 48.65 (pg/mL) TNFα Sandwich 6500 – 8.92 (pg/mL) 4522 (pg/mL) 11.5 (pg/mL) 7.74 (pg/mL) TNFα Sandwich 6500 – 8.92 (pg/mL) 4522 (pg/mL) 11.5 (pg/mL) 7.74 (pg/mL) TNFβ Sandwich 4000 – 5.49 (pg/mL) 2800 (pg/mL) 7.1 (pg/mL) 5.29 (pg/mL) TNFβ Sandwich 4000 – 5.49 (pg/mL) 2800 (pg/mL) 7.1 (pg/mL) 5.29 (pg/mL) Kit Components Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip well format please contact us for customization. Reagents Include: Calibrator: Lyophilized, recombinant antigens in a buffered protein base Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives Streptavidin HRP (1X) : Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase Wash Buffer (20X) : Liquid, 50 mL/vial of a concentrated solution of buffered surfactant Plate Seals (3) : Adhesive strips