Multiplex ELISA Kit For Rat Cytokine - Inflammation (9-plex)

Product Overview The Multiplex Rat Cytokine – Inflammation (9-Plex) is a fully quantitative ELISA-based Chemiluminescent assay allowing the concurrent measurement of nine biomarkers or analytes. Using just 25 µl of sample per well, up to 80 samples can be assayed for all nine markers in the panel within 3 hours. The Multiplex Rat Cytokine – Inflammation (9-Plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Multiplex plates are built by adsorbing nine distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results. Assay Ranges Analytes Calibrator Range Units LLD IFNγ 6000 – 8.2 pg/mL 5.3 IL-1α 5400 – 7.4 pg/mL 4.8 IL-1β 3200 – 4.4 pg/mL 2.9 IL-2 9800 – 13.4 pg/mL 8.7 IL-4 4000 – 5.5 pg/mL 3.6 IL-6 83000 – 113.9 pg/mL 74 IL-10 14000 – 19.2 pg/mL 12.5 IL-12p70 92000 – 126.2 pg/mL 82.0 TNFα 37000 – 50.8 pg/mL 33.0 Analytes Calibrator Range Units LLD Analytes Calibrator Range Units LLD Analytes Calibrator Range Units LLD IFNγ 6000 – 8.2 pg/mL 5.3 IL-1α 5400 – 7.4 pg/mL 4.8 IL-1β 3200 – 4.4 pg/mL 2.9 IL-2 9800 – 13.4 pg/mL 8.7 IL-4 4000 – 5.5 pg/mL 3.6 IL-6 83000 – 113.9 pg/mL 74 IL-10 14000 – 19.2 pg/mL 12.5 IL-12p70 92000 – 126.2 pg/mL 82.0 TNFα 37000 – 50.8 pg/mL 33.0 IFNγ 6000 – 8.2 pg/mL 5.3 IFNγ 6000 – 8.2 pg/mL 5.3 IL-1α 5400 – 7.4 pg/mL 4.8 IL-1α 5400 – 7.4 pg/mL 4.8 IL-1β 3200 – 4.4 pg/mL 2.9 IL-1β 3200 – 4.4 pg/mL 2.9 IL-2 9800 – 13.4 pg/mL 8.7 IL-2 9800 – 13.4 pg/mL 8.7 IL-4 4000 – 5.5 pg/mL 3.6 IL-4 4000 – 5.5 pg/mL 3.6 IL-6 83000 – 113.9 pg/mL 74 IL-6 83000 – 113.9 pg/mL 74 IL-10 14000 – 19.2 pg/mL 12.5 IL-10 14000 – 19.2 pg/mL 12.5 IL-12p70 92000 – 126.2 pg/mL 82.0 IL-12p70 92000 – 126.2 pg/mL 82.0 TNFα 37000 – 50.8 pg/mL 33.0 TNFα 37000 – 50.8 pg/mL 33.0 Kit Components Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip-well format, please contact us for customization. Reagents Include: Calibrator: Lyophilized, recombinant antigens in a buffered protein base Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives Streptavidin HRP (1X) : Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase Wash Buffer (20X) : Liquid, 50 mL/vial of a concentrated solution of buffered surfactant Plate Seals (3) : Adhesive strips