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B-Raf (Phospho-Thr753) Colorimetric Cell-Based ELISA Kit

The B-Raf (Phospho-Thr753) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor B-Raf (Phospho-Thr753) protein expression profile in cells. The kit can be used for measuring the relative amounts of B-Raf (Phospho-Thr753) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on B-Raf (Phospho-Thr753) .

Product Specifications

Synonyms

Serine/threonine-protein kinase B-raf;2.7.11.1; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1; BRAF; BRAF1, RAFB1

Gene Name

BRAF

UniProt

P15056

Reactivity

Human, Mouse, Rat

Tissue Specificity

Brain and testis.

Applications

ELISA

Detection Range

> 5000 cells/well

Function

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.

Molecular Weight

84437 MW

Shipping Conditions

Available

Storage Conditions

Store at 4°C for up to 6 months.

Other Gene Names

Serine/threonine-protein kinase B-raf

Subcellular Location

Nucleus. Cytoplasm. Cell membrane. Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.

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