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FOXO4 (Phospho-Ser262) Colorimetric Cell-Based ELISA Kit

The FOXO4 (Phospho-Ser262) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor FOXO4 (Phospho-Ser262) protein expression profile in cells. The kit can be used for measuring the relative amounts of FOXO4 (Phospho-Ser262) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on FOXO4 (Phospho-Ser262) .

Product Specifications

Synonyms

Forkhead box protein O4; Fork head domain transcription factor AFX1; FOXO4; AFX, AFX1, MLLT7

Gene Name

FOXO4

UniProt

P98177

Reactivity

Human, Mouse

Tissue Specificity

Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.

Applications

ELISA

Detection Range

> 5000 cells/well

Function

Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity.

Molecular Weight

53684 MW

Shipping Conditions

Available

Storage Conditions

Store at 4°C for up to 6 months.

Other Gene Names

Forkhead box protein O4

Subcellular Location

Cytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm.

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