HSP27 (Phospho-Ser82) Colorimetric Cell-Based ELISA Kit
The HSP27 (Phospho-Ser82) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor HSP27 (Phospho-Ser82) protein expression profile in cells. The kit can be used for measuring the relative amounts of HSP27 (Phospho-Ser82) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on HSP27 (Phospho-Ser82) .
Product Specifications
Synonyms
Heat shock protein beta-1; HspB1;28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27; HSPB1; HSP27, HSP28
Gene Name
HSPB1
UniProt
P04792
Reactivity
Human, Mouse, Rat
Tissue Specificity
Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
Applications
ELISA
Detection Range
> 5000 cells/well
Function
Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding- competent state (PubMed:10383393, PubMed:20178975) . Plays a role in stress resistance and actin organization (PubMed:19166925) . Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742) .
Molecular Weight
22783 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Product Datasheet
https://www.bosterbio.com/datasheet?sku=EKC2432
Product MSDS
https://www.bosterbio.com/msds?sku=EKC2432
Other Gene Names
Heat shock protein beta-1
Subcellular Location
Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub- nuclear structures known as SC35 speckles or nuclear splicing speckles.
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