Ezrin (Phospho-Tyr353) Colorimetric Cell-Based ELISA Kit

The Ezrin (Phospho-Tyr353) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Ezrin (Phospho-Tyr353) protein expression profile in cells. The kit can be used for measuring the relative amounts of Ezrin (Phospho-Tyr353) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Ezrin (Phospho-Tyr353) .

Product Specifications

Synonyms

Ezrin; Cytovillin; Villin-2; p81; EZR; VIL2

Gene Name

EZR

UniProt

P15311

Reactivity

Human, Mouse, Rat

Tissue Specificity

Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level) . Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied.

Applications

ELISA

Detection Range

> 5000 cells/well

Function

Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.

Molecular Weight

69413 MW

Shipping Conditions

Available

Storage Conditions

Store at 4°C for up to 6 months.

Product Datasheet

https://www.bosterbio.com/datasheet?sku=EKC2405

Product MSDS

https://www.bosterbio.com/msds?sku=EKC2405

Other Gene Names

Ezrin

Subcellular Location

Apical cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection. Cell projection, microvillus membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cell projection, microvillus. Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity) . Microvillar peripheral membrane protein (cytoplasmic side) .