ENOS (Phospho-Thr494) Colorimetric Cell-Based ELISA Kit
The eNOS (Phospho-Thr494) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor eNOS (Phospho-Thr494) protein expression profile in cells. The kit can be used for measuring the relative amounts of eNOS (Phospho-Thr494) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on eNOS (Phospho-Thr494) .
Product Specifications
Synonyms
Nitric oxide synthase, endothelial;1.14.13.39; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; N
Gene Name
NOS3
UniProt
P29474
Reactivity
Human, Mouse, Rat
Tissue Specificity
Platelets, placenta, liver and kidney.
Applications
ELISA
Detection Range
> 5000 cells/well
Function
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF) -induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Molecular Weight
133289 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
Nitric oxide synthase, endothelial
Subcellular Location
Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
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