EIF2alpha (Phospho-Ser51) Colorimetric Cell-Based ELISA Kit

The eIF2alpha (Phospho-Ser51) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor eIF2alpha (Phospho-Ser51) protein expression profile in cells. The kit can be used for measuring the relative amounts of eIF2alpha (Phospho-Ser51) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on eIF2alpha (Phospho-Ser51) .

Product Specifications

Synonyms

Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha; EIF2S1; EIF2A

Gene Name

EIF2S1

UniProt

P05198

Reactivity

Human, Mouse, Rat

Applications

ELISA

Detection Range

> 5000 cells/well

Function

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2- GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.