Cofilin (Phospho-Ser3) Colorimetric Cell-Based ELISA Kit

The Cofilin (Phospho-Ser3) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Cofilin (Phospho-Ser3) protein expression profile in cells. The kit can be used for measuring the relative amounts of Cofilin (Phospho-Ser3) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Cofilin (Phospho-Ser3) .

Product Specifications

Synonyms

Cofilin-1;18 kDa phosphoprotein; p18; Cofilin, non-muscle isoform; CFL1; CFL

Gene Name

CFL1

UniProt

P23528

Reactivity

Human, Mouse, Rat

Tissue Specificity

Widely distributed in various tissues.

Applications

ELISA

Detection Range

> 5000 cells/well

Function

Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (PubMed:11812157, PubMed:15580268, PubMed:21834987, PubMed:23633677) . Required for neural tube morphogenesis and neural crest cell migration (By similarity) .

Molecular Weight

18502 MW

Shipping Conditions

Available

Storage Conditions

Store at 4°C for up to 6 months.

Other Gene Names

Cofilin-1

Subcellular Location

Nucleus matrix. Cytoplasm, cytoskeleton. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium. Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Detected at the cleavage furrow and contractile ring during cytokinesis. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.