Vimentin (Phospho-Ser83) Colorimetric Cell-Based ELISA Kit

The Vimentin (Phospho-Ser83) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Vimentin (Phospho-Ser83) protein expression profile in cells. The kit can be used for measuring the relative amounts of Vimentin (Phospho-Ser83) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Vimentin (Phospho-Ser83) .

Product Specifications

Synonyms

Vimentin; VIM

Gene Name

VIM

UniProt

P08670

Reactivity

Human, Mouse, Rat

Tissue Specificity

Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines.

Applications

ELISA

Detection Range

> 5000 cells/well

Function

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.