Casein Kinase I alpha (Phospho-Tyr321) Colorimetric Cell-Based ELISA Kit

The Casein Kinase I alpha (Phospho-Tyr321) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Casein Kinase I alpha (Phospho-Tyr321) protein expression profile in cells. The kit can be used for measuring the relative amounts of Casein Kinase I alpha (Phospho-Tyr321) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Casein Kinase I alpha (Phospho-Tyr321) .

Product Specifications

Synonyms

Casein kinase I isoform alpha; CKI-alpha;2.7.11.1; CK1; CSNK1A1

Gene Name

CSNK1A1

UniProt

P48729

Reactivity

Human, Mouse, Rat

Applications

ELISA

Detection Range

> 5000 cells/well

Function

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis (PubMed:11955436, PubMed:1409656, PubMed:18305108) . May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration (PubMed:23902688) .

Molecular Weight

38915 MW

Shipping Conditions

Available

Storage Conditions

Store at 4°C for up to 6 months.

Other Gene Names

Casein kinase I isoform alpha

Subcellular Location

Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. Nucleus speckle. Cytoplasm, cytoskeleton, cilium basal body. Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis. Also recruited to the keratin cytoskeleton (PubMed:23902688) .