WEE1 (Phospho-Ser642) Colorimetric Cell-Based ELISA Kit
The WEE1 (Phospho-Ser642) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor WEE1 (Phospho-Ser642) protein expression profile in cells. The kit can be used for measuring the relative amounts of WEE1 (Phospho-Ser642) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on WEE1 (Phospho-Ser642) .
Product Specifications
Synonyms
WEE1hu; Wee1-like protein kinase; kinase WEE1
Gene Name
WEE1
UniProt
P30291
Reactivity
Human, Mouse, Rat
Applications
ELISA
Sample Type
Cell lines
Detection Range
> 5000 cells/well
Function
Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
Molecular Weight
71597 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
Wee1-like protein kinase
Subcellular Location
Nucleus.
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