TISB (Phospho-Ser92) Colorimetric Cell-Based ELISA Kit

Expressed mainly in the basal epidermal layer, weakly in the suprabasal epidermal layers (PubMed:27182009) . Expressed in epidermal keratinocytes (at protein level) (PubMed:27182009) . Expressed in osteoblasts (PubMed:15465005) .

Zinc-finger RNA-binding protein that destabilizes several cytoplasmic AU-rich element (ARE) -containing mRNA transcripts by promoting their poly (A) tail removal or deadenylation, and hence provide a mechanism for attenuating protein synthesis (PubMed:12198173, PubMed:15538381, PubMed:15467755, PubMed:17030608, PubMed:19179481, PubMed:20702587, PubMed:24700863, PubMed:25106868, PubMed:25014217, PubMed:26542173) . Acts as a 3'-untranslated region (UTR) ARE mRNA-binding adapter protein to communicate signaling events to the mRNA decay machinery (PubMed:15687258) . Functions by recruiting the CCR4-NOT deadenylase complex and components of the cytoplasmic RNA decay machinery to the bound ARE-containing mRNAs, and hence promotes ARE-mediated mRNA deadenylation and decay processes (PubMed:15687258, PubMed:18326031, PubMed:25106868) . Induces also the degradation of ARE-containing mRNAs even in absence of poly (A) tail (By similarity) . Binds to 3'-UTR ARE of numerous mRNAs (PubMed:12198173, PubMed:15538381, PubMed:15467755, PubMed:17030608, PubMed:19179481, PubMed:20702587, PubMed:24700863, PubMed:25106868, PubMed:25014217, PubMed:26542173) . Positively regulates early adipogenesis by promoting ARE-mediated mRNA decay of immediate early genes (IEGs) (By similarity) . Promotes ARE-mediated mRNA decay of mineralocorticoid receptor NR3C2 mRNA in response to hypertonic stress (PubMed:24700863) . Negatively regulates hematopoietic/erythroid cell differentiation by promoting ARE- mediated mRNA decay of the transcription factor STAT5B mRNA (PubMed:20702587) . Positively regulates monocyte/macrophage cell differentiation by promoting ARE-mediated mRNA decay of the cyclin-dependent kinase CDK6 mRNA (PubMed:26542173) . Promotes degradation of ARE-containing pluripotency-associated mRNAs in embryonic stem cells (ESCs), such as NANOG, through a fibroblast growth factor (FGF) -induced MAPK-dependent signaling pathway, and hence attenuates ESC self-renewal and positively regulates mesendoderm differentiation (By similarity) . May play a role in mediating pro-apoptotic effects in malignant B-cells by promoting ARE-mediated mRNA decay of BCL2 mRNA (PubMed:25014217) . In association with ZFP36L2 maintains quiescence on developing B lymphocytes by promoting ARE-mediated decay of several mRNAs encoding cell cycle regulators that help B cells progress through the cell cycle, and hence ensuring accurate variable-diversity- joining (VDJ) recombination and functional immune cell formation (By similarity) . Together with ZFP36L2 is also necessary for thymocyte development and prevention of T-cell acute lymphoblastic leukemia (T-ALL) transformation by promoting ARE-mediated mRNA decay of the oncogenic transcription factor NOTCH1 mRNA (By similarity) . Participates in the delivery of target ARE-mRNAs to processing bodies (PBs) (PubMed:17369404) . In addition to its cytosolic mRNA-decay function, plays a role in the regulation of nuclear mRNA 3'-end processing; modulates mRNA 3'-end maturation efficiency of the DLL4 mRNA through binding with an ARE embedded in a weak noncanonical polyadenylation (poly (A) ) signal in endothelial cells (PubMed:21832157) . Also involved in the regulation of stress granule (SG) and P-body (PB) formation and fusion (PubMed:15967811) . Plays a role in vasculogenesis and endocardial development (By similarity) . Plays a role in the regulation of keratinocyte proliferation, differentiation and apoptosis (PubMed:27182009) . Plays a role in myoblast cell differentiation (By similarity) .

Nucleus. Cytoplasm. Cytoplasmic granule. Cytoplasm, P-body. Shuttles between the nucleus and the cytoplasm in a XPO1/CRM1-dependent manner (By similarity) . Component of cytoplasmic stress granules (PubMed:15967811) . Localizes in processing bodies (PBs) (PubMed:17369404) .