PDCD4 (Phospho-Ser457) Colorimetric Cell-Based ELISA Kit

The PDCD4 (Phospho-Ser457) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor PDCD4 (Phospho-Ser457) protein expression profile in cells. The kit can be used for measuring the relative amounts of PDCD4 (Phospho-Ser457) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on PDCD4 (Phospho-Ser457) .

Product Specifications

Synonyms

Programmed cell death protein 4; Nuclear antigen H731-like; Neoplastic transformation inhibitor protein; Protein 197/15a; PDCD4; H731

Gene Name

PDCD4

UniProt

Q53EL6

Reactivity

Human, Mouse, Rat

Tissue Specificity

Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.

Applications

ELISA

Sample Type

Cell lines

Detection Range

> 5000 cells/well

Function

Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA (By similarity) .

Molecular Weight

51735 MW

Shipping Conditions

Available

Storage Conditions

Store at 4°C for up to 6 months.

Other Gene Names

Programmed cell death protein 4

Subcellular Location

Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm (By similarity) . Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457 (PubMed:16357133) .