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ENOS (Phospho-Ser615) Colorimetric Cell-Based ELISA Kit

The eNOS (Phospho-Ser615) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor eNOS (Phospho-Ser615) protein expression profile in cells. The kit can be used for measuring the relative amounts of eNOS (Phospho-Ser615) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on eNOS (Phospho-Ser615) .

Product Specifications

Synonyms

CNOS; Constitutive NOS; EC-NOS; ECNOS; Endothelial NOS; Nitric-oxide synthase, endothelial; NOS, type III; NOS3; NOSIII

Gene Name

NOS3

UniProt

P29474

Reactivity

Human, Mouse, Rat

Tissue Specificity

Platelets, placenta, liver and kidney.

Applications

ELISA

Sample Type

Cell lines

Detection Range

> 5000 cells/well

Function

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF) -induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.

Molecular Weight

133289 MW

Shipping Conditions

Available

Storage Conditions

Store at 4°C for up to 6 months.

Other Gene Names

Nitric oxide synthase, endothelial

Subcellular Location

Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.

Frequently Asked Questions

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