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IkappaB-alpha (Phospho-Tyr305) Colorimetric Cell-Based ELISA Kit

The IkappaB-alpha (Phospho-Tyr305) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor IkappaB-alpha (Phospho-Tyr305) protein expression profile in cells. The kit can be used for measuring the relative amounts of IkappaB-alpha (Phospho-Tyr305) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on IkappaB-alpha (Phospho-Tyr305) .

Product Specifications

Synonyms

I-kappa-B-alpha; IkappaBalpha; IKBA; MAD3; Major histocompatibility complex enhancer-binding protein MAD3; NF-kappaB inhibitor alpha; NFKBI; NFKBIA; RL/IF-1

Gene Name

NFKBIA

UniProt

P25963

Reactivity

Human, Mouse, Rat

Applications

ELISA

Sample Type

Cell lines

Detection Range

> 5000 cells/well

Function

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.

Molecular Weight

35609 MW

Shipping Conditions

Available

Storage Conditions

Store at 4°C for up to 6 months.

Other Gene Names

NF-kappa-B inhibitor alpha

Subcellular Location

Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.

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