Smad1 (Phospho-Ser187) Colorimetric Cell-Based ELISA Kit

The Smad1 (Phospho-Ser187) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Smad1 (Phospho-Ser187) protein expression profile in cells. The kit can be used for measuring the relative amounts of Smad1 (Phospho-Ser187) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Smad1 (Phospho-Ser187) .

Product Specifications

Synonyms

BSP-1; BSP1; Dwarfin-A; Dwf-A; JV4-1; MADH1; MADR1; Mad-related protein 1; Mad1; Mothers against DPP homolog 1; Mothers against decapentaplegic homolog 1; Mothers-against-DPP-related-1; SMAD 1; Smad 1; Transforming growth factor- beta signaling protein-1

Gene Name

SMAD1

UniProt

Q15797

Reactivity

Human, Mouse

Tissue Specificity

Ubiquitous. Highest expression seen in the heart and skeletal muscle.

Applications

ELISA

Sample Type

Cell lines

Detection Range

> 5000 cells/well

Function

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD) . SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP) -mediated cardiac-specific gene expression.

Molecular Weight

52260 MW

Shipping Conditions

Available

Storage Conditions

Store at 4°C for up to 6 months.

Other Gene Names

Mothers against decapentaplegic homolog 1

Subcellular Location

Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4 (PubMed:15647271) . Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15647271) . Exported from the nucleus to the cytoplasm when dephosphorylated (By similarity) .