MCM4 (Phospho-Ser54) Colorimetric Cell-Based ELISA Kit

The MCM4 (Phospho-Ser54) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor MCM4 (Phospho-Ser54) protein expression profile in cells. The kit can be used for measuring the relative amounts of MCM4 (Phospho-Ser54) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on MCM4 (Phospho-Ser54) .

Product Specifications

Synonyms

CDC21 homolog; DNA replication licensing factor MCM4; P1-CDC21

Gene Name

MCM4

UniProt

P33991

Reactivity

Human, Mouse

Applications

ELISA

Sample Type

Cell lines

Detection Range

> 5000 cells/well

Function

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.