53BP1 (Phospho-Ser6) Colorimetric Cell-Based ELISA Kit
The 53BP1 (Phospho-Ser6) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor 53BP1 (Phospho-Ser6) protein expression profile in cells. The kit can be used for measuring the relative amounts of 53BP1 (Phospho-Ser6) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on 53BP1 (Phospho-Ser6) .
Product Specifications
Synonyms
P53-binding protein 1; P531; TP53B; TP53BP1; TRP53BP1; Tumor suppressor p53-binding protein 1
Gene Name
TP53BP1
UniProt
Q12888
Reactivity
Human, Mouse, Rat
Applications
ELISA
Sample Type
Cell lines
Detection Range
> 5000 cells/well
Function
Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis (PubMed:12364621, PubMed:22553214, PubMed:23333306, PubMed:17190600, PubMed:21144835, PubMed:28241136) . Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ) -mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1 (PubMed:22553214, PubMed:23727112, PubMed:23333306) . In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites (PubMed:28241136) . Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites (PubMed:23760478, PubMed:28241136, PubMed:17190600) . Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs (PubMed:23345425) . Participates to the repair and the orientation of the broken DNA ends during CSR (By similarity) . In contrast, it is not required for classic NHEJ and V (D) J recombination (By similarity) . Promotes NHEJ of dysfunctional telomeres via interaction with PAXIP1 (PubMed:23727112) .
Molecular Weight
213574 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
TP53-binding protein 1
Subcellular Location
Nucleus. Chromosome. Chromosome, centromere, kinetochore. Localizes to the nucleus in absence of DNA damage (PubMed:28241136) . Following DNA damage, recruited to sites of DNA damage, such as double stand breaks (DSBs) : recognizes and binds histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites (PubMed:23333306, PubMed:23760478, PubMed:24703952, PubMed:28241136, PubMed:17190600) . Associated with kinetochores during mitosis (By similarity) .
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
