SP1 (Phospho-Thr453) Colorimetric Cell-Based ELISA Kit

The SP1 (Phospho-Thr453) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor SP1 (Phospho-Thr453) protein expression profile in cells. The kit can be used for measuring the relative amounts of SP1 (Phospho-Thr453) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on SP1 (Phospho-Thr453) .

Product Specifications

Synonyms

TSFP1; Transcription factor SP1; transcription factor Sp1

Gene Name

SP1

UniProt

P08047

Reactivity

Human, Mouse, Rat

Tissue Specificity

Up-regulated in adenocarcinomas of the stomach (at protein level) . Isoform 3 is ubiquitously expressed at low levels.

Applications

ELISA

Sample Type

Cell lines

Detection Range

> 5000 cells/well

Function

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation) . Binds also the PDGFR- alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression. In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression. Isoform 3 is a stronger activator of transcription than isoform 1. Positively regulates the transcription of the core clock component ARNTL/BMAL1.

Molecular Weight

80693 MW

Shipping Conditions

Available

Storage Conditions

Store at 4°C for up to 6 months.

Product Datasheet

https://www.bosterbio.com/datasheet?sku=EKC2122

Product MSDS

https://www.bosterbio.com/msds?sku=EKC2122

Other Gene Names

Transcription factor Sp1

Subcellular Location

Nucleus. Cytoplasm. Nuclear location is governed by glycosylated/phosphorylated states. Insulin promotes nuclear location, while glucagon favors cytoplasmic location.