SMC1 (Phospho-Ser966) Colorimetric Cell-Based ELISA Kit

The SMC1 (Phospho-Ser966) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor SMC1 (Phospho-Ser966) protein expression profile in cells. The kit can be used for measuring the relative amounts of SMC1 (Phospho-Ser966) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on SMC1 (Phospho-Ser966) .

Product Specifications

Synonyms

Chromosome segregation protein SmcB; DXS423E; KIAA0178; SB1.8/DXS423E protein; SM1A; SMC1A; SMC1L1; SMC1alpha protein; Sb1.8; Structural maintenance of chromosome 1-like 1 protein

Gene Name

SMC1A

UniProt

Q14683

Reactivity

Human, Mouse, Rat

Applications

ELISA

Sample Type

Cell lines

Detection Range

> 5000 cells/well

Function

Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.

Molecular Weight

143233 MW

Shipping Conditions

Available

Storage Conditions

Store at 4°C for up to 6 months.

Other Gene Names

Structural maintenance of chromosomes protein 1A

Subcellular Location

Nucleus. Chromosome. Chromosome, centromere, kinetochore. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex. The phosphorylated form on Ser-957 and Ser-966 associates with chromatin during G1/S/G2 phases but not during M phase, suggesting that phosphorylation does not regulate cohesin function. Integral component of the functional centromere- kinetochore complex at the kinetochore region during mitosis.