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PP2A-alpha (Phospho-Tyr307) Colorimetric Cell-Based ELISA Kit

The PP2A-alpha (Phospho-Tyr307) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor PP2A-alpha (Phospho-Tyr307) protein expression profile in cells. The kit can be used for measuring the relative amounts of PP2A-alpha (Phospho-Tyr307) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on PP2A-alpha (Phospho-Tyr307) .

Product Specifications

Synonyms

PP2A-alpha; PP2AA; RP-C; Replication protein C; Serine/threonine protein phosphatase 2A; catalytic subunit, alpha isoform

Gene Name

PPP2CA

UniProt

P67775

Reactivity

Human, Mouse, Rat

Applications

ELISA

Sample Type

Cell lines

Detection Range

> 5000 cells/well

Function

PP2A is the major phosphatase for microtubule-associated proteins (MAPs) . PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGO2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity) . Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'.

Molecular Weight

35594 MW

Shipping Conditions

Available

Storage Conditions

Store at 4°C for up to 6 months.

Other Gene Names

Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

Subcellular Location

Cytoplasm. Nucleus. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGO2 (By similarity) .

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