PDE4D (Phospho-Ser190/53) Colorimetric Cell-Based ELISA Kit

The PDE4D (Phospho-Ser190/53) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor PDE4D (Phospho-Ser190/53) protein expression profile in cells. The kit can be used for measuring the relative amounts of PDE4D (Phospho-Ser190/53) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on PDE4D (Phospho-Ser190/53) .

Product Specifications

Synonyms

DPDE3, PDE43, PDE4D4, cAMP-specific 3',5'-cyclic phosphodiesterase 4D

Gene Name

PDE4D

UniProt

Q08499

Reactivity

Human, Mouse, Rat

Tissue Specificity

Expressed in colonic epithelial cells (at protein level) . Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.

Applications

ELISA

Sample Type

Cell lines

Detection Range

> 5000 cells/well

Function

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.

Molecular Weight

91115 MW

Shipping Conditions

Available

Storage Conditions

Store at 4°C for up to 6 months.

Other Gene Names

CAMP-specific 3',5'-cyclic phosphodiesterase 4D

Subcellular Location

Apical cell membrane. Cytoplasm. Membrane. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome (By similarity) . Colocalized with SHANK2 to the apical membrane of colonic crypt cells.