NNOS (Phospho-Ser852) Colorimetric Cell-Based ELISA Kit
The nNOS (Phospho-Ser852) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor nNOS (Phospho-Ser852) protein expression profile in cells. The kit can be used for measuring the relative amounts of nNOS (Phospho-Ser852) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on nNOS (Phospho-Ser852) .
Product Specifications
Synonyms
BNOS; BNOS; Constitutive NOS; N-NOS; NC-NOS; Neuronal NOS; Nitric-oxide synthase brain; NOS type I; NOS1
Gene Name
NOS1
UniProt
P29475
Reactivity
Human, Mouse, Rat
Tissue Specificity
Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.
Applications
ELISA
Sample Type
Cell lines
Detection Range
> 5000 cells/well
Function
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
Molecular Weight
160970 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
Nitric oxide synthase, brain
Subcellular Location
Cell membrane, sarcolemma; Peripheral membrane protein. Cell projection, dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines (By similarity) .
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