HDAC5 (Phospho-Ser259) Colorimetric Cell-Based ELISA Kit
The HDAC5 (Phospho-Ser259) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor HDAC5 (Phospho-Ser259) protein expression profile in cells. The kit can be used for measuring the relative amounts of HDAC5 (Phospho-Ser259) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on HDAC5 (Phospho-Ser259) .
Product Specifications
Synonyms
Antigen NY-CO-9; HD5; Histone deacetylase 5; Histone deacetylase mHDA1; KIAA0600
Gene Name
HDAC5
UniProt
Q9UQL6
Reactivity
Human, Mouse
Tissue Specificity
Ubiquitous.
Applications
ELISA
Sample Type
Cell lines
Detection Range
> 5000 cells/well
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) . Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer.
Molecular Weight
121978 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
Histone deacetylase 5
Subcellular Location
Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by AMPK, CaMK1 and SIK1.
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