E2F1 (Phospho-Thr433) Colorimetric Cell-Based ELISA Kit
The E2F1 (Phospho-Thr433) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor E2F1 (Phospho-Thr433) protein expression profile in cells. The kit can be used for measuring the relative amounts of E2F1 (Phospho-Thr433) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on E2F1 (Phospho-Thr433) .
Product Specifications
Synonyms
E2F-1; PBR3; PRB-binding protein E2F-1; RBAP-1; RBBP-3; RBBP3; Retinoblastoma binding protein 3; Retinoblastoma-associated protein 1; Transcription factor E2F1
Gene Name
E2F1
UniProt
Q01094
Reactivity
Human, Mouse
Applications
ELISA
Sample Type
Cell lines
Detection Range
> 5000 cells/well
Function
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters (PubMed:20176812) . Positively regulates transcription of RRP1B (PubMed:20040599) .
Molecular Weight
46920 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
Transcription factor E2F1
Subcellular Location
Nucleus.
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