WNK1 (Phospho-Thr58) Colorimetric Cell-Based ELISA Kit
The WNK1 (Phospho-Thr58) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor WNK1 (Phospho-Thr58) protein expression profile in cells. The kit can be used for measuring the relative amounts of WNK1 (Phospho-Thr58) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on WNK1 (Phospho-Thr58) .
Product Specifications
Synonyms
WNK lysine deficient protein kinase 1; KDP; PRKWNK1; PROTEIN KINASE; LYSINE-DEFICIENT 1
Gene Name
WNK1 HSN2 , KDP, KIAA0344 , PRKWNK1
UniProt
Q9H4A3
Reactivity
Human, Mouse, Rat
Tissue Specificity
Widely expressed, with highest levels observed in the testis, heart, kidney and skeletal muscle. Isoform 3 is kidney-specific and specifically expressed in the distal convoluted tubule (DCT) and connecting tubule (CNT) of the nephron.
Applications
ELISA
Sample Type
Cell lines
Detection Range
> 5000 cells/well
Function
Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide- sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition (By similarity) .
Molecular Weight
250794 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
Serine/threonine-protein kinase WNK1
Subcellular Location
Cytoplasm.
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