STAT2 (Phospho-Tyr690) Colorimetric Cell-Based ELISA Kit

The STAT2 (Phospho-Tyr690) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor STAT2 (Phospho-Tyr690) protein expression profile in cells. The kit can be used for measuring the relative amounts of STAT2 (Phospho-Tyr690) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on STAT2 (Phospho-Tyr690) .

Product Specifications

Synonyms

Signal transducer and activator of transcription 2

Gene Name

STAT2

UniProt

P52630

Reactivity

Human, Rat

Applications

ELISA

Sample Type

Cell lines

Detection Range

> 5000 cells/well

Function

Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta) . Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state (PubMed:9020188, PubMed:23391734) . Acts as a regulator of mitochondrial fission by modulating the phosphorylation of DNM1L at 'Ser-616' and 'Ser-637' which activate and inactivate the GTPase activity of DNM1L respectively (PubMed:26122121) .