Caspase 8 (Phospho-Ser347) Colorimetric Cell-Based ELISA Kit

The Caspase 8 (Phospho-Ser347) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Caspase 8 (Phospho-Ser347) protein expression profile in cells. The kit can be used for measuring the relative amounts of Caspase 8 (Phospho-Ser347) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Caspase 8 (Phospho-Ser347) .

Product Specifications

Synonyms

Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; Caspase-8 precursor; FADD-homologous ICE/CED-3- like protease; FADD-like ICE; FLICE; ICE-like apoptotic protease 5; ICE8; MACH; MCH5; MORT1-associated CED-3 homolog

Gene Name

CASP8

UniProt

Q14790

Reactivity

Human, Rat

Tissue Specificity

Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle.

Applications

ELISA

Sample Type

Cell lines

Detection Range

> 5000 cells/well

Function

Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death- inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.