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Cytochrome P450 7B1 Colorimetric Cell-Based ELISA

The Cytochrome P450 7B1 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Cytochrome P450 7B1 protein expression profile in cells. The kit can be used for measuring the relative amounts of Cytochrome P450 7B1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Cytochrome P450 7B1.

Product Specifications

Synonyms

25-hydroxycholesterol 7-alpha-hydroxylase;1.14.14.29 ; Cytochrome P450 7B1; Oxysterol 7-alpha-hydroxylase; CYP7B1

Gene Name

CYP7B1

UniProt

O75881

Reactivity

Human

Tissue Specificity

Brain, testis, ovary, prostate, liver, colon, kidney, and small intestine.

Applications

ELISA

Detection Range

> 5000 cells/well

Function

Oxysterol 7alpha-hydroxylase that mediates formation of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC) from 25- hydroxycholesterol (PubMed:10588945) . Plays a key role in cell positioning and movement in lymphoid tissues: 7-alpha,25- dihydroxycholesterol (7-alpha,25-OHC) acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells (By similarity) .

Molecular Weight

58256 MW

Shipping Conditions

Available

Storage Conditions

Store at 4°C for up to 6 months.

Other Gene Names

25-hydroxycholesterol 7-alpha-hydroxylase

Subcellular Location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Frequently Asked Questions

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