Cytochrome P450 3A4/5 Colorimetric Cell-Based ELISA

Cytochrome P450 3A4;1.14.13.-;1,8-cineole 2-exo-monooxygenase;1.14.13.157; Albendazole monooxygenase;1.14.13.32; Albendazole sulfoxidase; CYPIIIA3; CYPIIIA4; Cholesterol 25-hydroxylase ;1.14.14.1 ; Cytochrome P450 3A3; Cytochrome P450 HLp; Cytochrome P450 NF-25; Cytochrome P450-PCN1; Nifedipine oxidase; Quinine 3-monooxygenase;1.14.13.67; Taurochenodeoxycholate 6-alpha-hydroxylase;1.14.13.97; CYP3A4; CYP3A3

Expressed in prostate and liver. According to some authors, it is not expressed in brain (PubMed:19094056) . According to others, weak levels of expression are measured in some brain locations (PubMed:19359404 and PubMed:18545703) . Also expressed in epithelium of the small intestine and large intestine, bile duct, nasal mucosa, kidney, adrenal cortex, epithelium of the gastric mucosa with intestinal metaplasia, gallbladder, intercalated ducts of the pancreas, chief cells of the parathyroid and the corpus luteum of the ovary (at protein level) .

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4- hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,8-cineole 2- exo-monooxygenase. The enzyme also hydroxylates etoposide (PubMed:11159812) . Catalyzes 4-beta-hydroxylation of cholesterol. May catalyze 25-hydroxylation of cholesterol in vitro (PubMed:21576599) .