MADD Colorimetric Cell-Based ELISA

The MADD Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor MADD protein expression profile in cells. The kit can be used for measuring the relative amounts of MADD in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on MADD.

Product Specifications

Synonyms

MAP kinase-activating death domain protein; Differentially expressed in normal and neoplastic cells; Insulinoma glucagonoma clone 20; Rab3 GDP/GTP exchange factor; MADD ; DENN , IG20 , KIAA0358

Gene Name

MADD DENN , IG20 , KIAA0358

UniProt

Q8WXG6

Reactivity

Human, Mouse, Rat

Tissue Specificity

Highly expressed in fetal brain and kidney; adult testis, ovary, brain and heart. Isoform 5 is constitutively expressed in all tissues. Isoform 7 is expressed in fetal liver and in several cancer cell lines.

Applications

ELISA

Detection Range

> 5000 cells/well

Function

Plays a significant role in regulating cell proliferation, survival and death through alternative mRNA splicing. Isoform 5 shows increased cell proliferation and isoform 2 shows decreased. Converts GDP-bound inactive form of RAB3A, RAB3C and RAB3D to the GTP-bound active forms. Component of the TNFRSF1A signaling complex: MADD links TNFRSF1A with MAP kinase activation. Plays an important regulatory role in physiological cell death (TNF-alpha-induced, caspase-mediated apoptosis) ; isoform 1 is susceptible to inducing apoptosis, isoform 5 is resistant and isoform 3 and isoform 4 have no effect.