KSR2 Colorimetric Cell-Based ELISA

The KSR2 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor KSR2 protein expression profile in cells. The kit can be used for measuring the relative amounts of KSR2 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on KSR2.

Product Specifications

Synonyms

Kinase suppressor of Ras 2; hKSR2;2.7.11.1; KSR2

Gene Name

KSR2

UniProt

Q6VAB6

Reactivity

Human, Mouse

Tissue Specificity

Mainly expressed in brain and kidney.

Applications

ELISA

Detection Range

> 5000 cells/well

Function

Location-regulated scaffold connecting MEK to RAF. Has very low protein kinase activity and can phosphorylate MAP2K1 at several Ser and Thr residues with very low efficiency (in vitro) . Interaction with BRAF enhances KSR2-mediated phosphorylation of MAP2K1 (in vitro) . Blocks MAP3K8 kinase activity and MAP3K8- mediated signaling. Acts as a negative regulator of MAP3K3- mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production.