WTAP Colorimetric Cell-Based ELISA

The WTAP Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor WTAP protein expression profile in cells. The kit can be used for measuring the relative amounts of WTAP in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on WTAP.

Product Specifications

Synonyms

Pre-mRNA-splicing regulator WTAP ; Female-lethal (2) D homolog ; hFL (2) D ; WT1-associated protein ; Wilms tumor 1-associating protein ; WTAP ; KIAA0105

Gene Name

WTAP KIAA0105

UniProt

Q15007

Reactivity

Human, Mouse

Tissue Specificity

Ubiquitously expressed.

Applications

ELISA

Detection Range

> 5000 cells/well

Function

Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (PubMed:29507755) . Required for accumulation of METTL3 and METTL14 to nuclear speckle (PubMed:24316715, PubMed:24407421, PubMed:24981863) . Acts as a mRNA splicing regulator (PubMed:12444081) . Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability (PubMed:17088532) . Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes (PubMed:17095724) .

Molecular Weight

44244 MW

Shipping Conditions

Available

Storage Conditions

Store at 4°C for up to 6 months.

Product Datasheet

https://www.bosterbio.com/datasheet?sku=EKC1815

Product MSDS

https://www.bosterbio.com/msds?sku=EKC1815

Other Gene Names

Pre-mRNA-splicing regulator WTAP

Subcellular Location

Nucleus speckle. Nucleus, nucleoplasm. Cytoplasm. Mainly nuclear with some fraction located in the cytoplasm. ZC3H13 is required to anchor component of the MACOM subcomplex, such as VIRMA, in the nucleus.