WTAP Colorimetric Cell-Based ELISA
The WTAP Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor WTAP protein expression profile in cells. The kit can be used for measuring the relative amounts of WTAP in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on WTAP.
Product Specifications
Synonyms
Pre-mRNA-splicing regulator WTAP ; Female-lethal (2) D homolog ; hFL (2) D ; WT1-associated protein ; Wilms tumor 1-associating protein ; WTAP ; KIAA0105
Gene Name
WTAP KIAA0105
UniProt
Q15007
Reactivity
Human, Mouse
Tissue Specificity
Ubiquitously expressed.
Applications
ELISA
Detection Range
> 5000 cells/well
Function
Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (PubMed:29507755) . Required for accumulation of METTL3 and METTL14 to nuclear speckle (PubMed:24316715, PubMed:24407421, PubMed:24981863) . Acts as a mRNA splicing regulator (PubMed:12444081) . Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability (PubMed:17088532) . Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes (PubMed:17095724) .
Molecular Weight
44244 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
Pre-mRNA-splicing regulator WTAP
Subcellular Location
Nucleus speckle. Nucleus, nucleoplasm. Cytoplasm. Mainly nuclear with some fraction located in the cytoplasm. ZC3H13 is required to anchor component of the MACOM subcomplex, such as VIRMA, in the nucleus.
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