MELK Colorimetric Cell-Based ELISA

The MELK Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor MELK protein expression profile in cells. The kit can be used for measuring the relative amounts of MELK in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on MELK.

Product Specifications

Synonyms

Maternal embryonic leucine zipper kinase; hMELK;2.7.11.1; Protein kinase Eg3; pEg3 kinase; Protein kinase PK38; hPK38; Tyrosine-protein kinase MELK;2.7.10.2; MELK; KIAA0175

Gene Name

MELK

UniProt

Q14680

Reactivity

Human

Tissue Specificity

Expressed in placenta, kidney, thymus, testis, ovary and intestine.

Applications

ELISA

Detection Range

> 5000 cells/well

Function

Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.