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Glucokinase Regulator Colorimetric Cell-Based ELISA

The Glucokinase Regulator Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Glucokinase Regulator protein expression profile in cells. The kit can be used for measuring the relative amounts of Glucokinase Regulator in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Glucokinase Regulator.

Product Specifications

Synonyms

Glucokinase regulatory protein; GKRP; Glucokinase regulator; GCKR

Gene Name

GCKR

UniProt

Q14397

Reactivity

Human, Mouse, Rat

Tissue Specificity

Found in liver and pancreas. Not detected in muscle, brain, heart, thymus, intestine, uterus, adipose tissue, kidney, adrenal, lung or spleen.

Applications

ELISA

Detection Range

> 5000 cells/well

Function

Inhibits glucokinase (GCK) by forming an inactive complex with this enzyme. The affinity of GCKR for GCK is modulated by fructose metabolites: GCKR with bound fructose 6- phosphate has increased affinity for GCK, while GCKR with bound fructose 1-phosphate has strongly decreased affinity for GCK and does not inhibit GCK activity.

Molecular Weight

68685 MW

Shipping Conditions

Available

Storage Conditions

Store at 4°C for up to 6 months.

Other Gene Names

Glucokinase regulatory protein

Subcellular Location

Cytoplasm. Nucleus. Under low glucose concentrations, GKRP associates with GCK and the inactive complex is recruited to the hepatocyte nucleus.

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