DMPK Colorimetric Cell-Based ELISA
The DMPK Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor DMPK protein expression profile in cells. The kit can be used for measuring the relative amounts of DMPK in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on DMPK.
Product Specifications
Synonyms
Myotonin-protein kinase; MT-PK;2.7.11.1; DM-kinase; DMK; DM1 protein kinase; DMPK; Myotonic dystrophy protein kinase; DMPK; DM1PK, MDPK
Gene Name
DMPK
UniProt
Q09013
Reactivity
Human
Tissue Specificity
Most isoforms are expressed in many tissues including heart, skeletal muscle, liver and brain, except for isoform 2 which is only found in the heart and skeletal muscle, and isoform 14 which is only found in the brain, with high levels in the striatum, cerebellar cortex and pons.
Applications
ELISA
Detection Range
> 5000 cells/well
Function
Non-receptor serine/threonine protein kinase which is necessary for the maintenance of skeletal muscle structure and function. May play a role in myocyte differentiation and survival by regulating the integrity of the nuclear envelope and the expression of muscle-specific genes. May also phosphorylate PPP1R12A and inhibit the myosin phosphatase activity to regulate myosin phosphorylation. Also critical to the modulation of cardiac contractility and to the maintenance of proper cardiac conduction activity probably through the regulation of cellular calcium homeostasis. Phosphorylates PLN, a regulator of calcium pumps and may regulate sarcoplasmic reticulum calcium uptake in myocytes. May also phosphorylate FXYD1/PLM which is able to induce chloride currents. May also play a role in synaptic plasticity.
Molecular Weight
69385 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
Myotonin-protein kinase
Subcellular Location
Endoplasmic reticulum membrane; Single-pass type IV membrane protein; Cytoplasmic side. Nucleus outer membrane; Single-pass type IV membrane protein; Cytoplasmic side. Mitochondrion outer membrane; Single-pass type IV membrane protein. Sarcoplasmic reticulum membrane. Cell membrane. Cytoplasm, cytosol. Localizes to sarcoplasmic reticulum membranes of cardiomyocytes.
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