ST5 Colorimetric Cell-Based ELISA

The ST5 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor ST5 protein expression profile in cells. The kit can be used for measuring the relative amounts of ST5 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on ST5.

Product Specifications

Synonyms

Suppression of tumorigenicity 5 protein; DENN domain-containing protein 2B; HeLa tumor suppression 1; ST5; DENND2B, HTS1

Gene Name

ST5

UniProt

P78524

Reactivity

Human, Mouse

Tissue Specificity

Widely expressed with the exception of peripheral blood lymphocytes. Isoform 1 is expressed in several epithelial and fibroblast (including tumorigenic) but absent in lymphoid cell lines (at protein level) . Isoform 3 is expressed in primary cell or weakly tumorigenic but not in tumorigenic cell lines (at protein level) .

Applications

ELISA

Detection Range

> 5000 cells/well

Function

Guanine nucleotide exchange factor (GEF) which may activate RAB9A and RAB9B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP- bound form. May be involved in cytoskeletal organization and tumorogenicity. Isoform 1 seems to be involved in a signaling transduction pathway leading to activation of MAPK1/ERK2. Isoform 3 may block ERK2 activation stimulated by ABL1. Isoform 3 may alter cell morphology and cell growth.