MAT1 Colorimetric Cell-Based ELISA

The MAT1 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor MAT1 protein expression profile in cells. The kit can be used for measuring the relative amounts of MAT1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on MAT1.

Product Specifications

Synonyms

CDK-activating kinase assembly factor MAT1; CDK7/cyclin-H assembly factor; Cyclin-G1-interacting protein; Menage a trois; RING finger protein 66; RING finger protein MAT1; p35; p36; MNAT1; CAP35, MAT1, RNF66

Gene Name

MNAT1

UniProt

P51948

Reactivity

Human, Mouse, Rat

Tissue Specificity

Highest levels in colon and testis. Moderate levels are present thymus, prostate, ovary, and small intestine. The lowest levels are found in spleen and leukocytes.

Applications

ELISA

Detection Range

> 5000 cells/well

Function

Stabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II.