CLK2 Colorimetric Cell-Based ELISA

The CLK2 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor CLK2 protein expression profile in cells. The kit can be used for measuring the relative amounts of CLK2 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on CLK2.

Product Specifications

Synonyms

Dual specificity protein kinase CLK2;2.7.12.1; CDC-like kinase 2; CLK2

Gene Name

CLK2

UniProt

P49760

Reactivity

Human, Mouse, Rat

Tissue Specificity

Endothelial cells.

Applications

ELISA

Detection Range

> 5000 cells/well

Function

Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing and can cause redistribution of SR proteins from speckles to a diffuse nucleoplasmic distribution. Acts as a suppressor of hepatic gluconeogenesis and glucose output by repressing PPARGC1A transcriptional activity on gluconeogenic genes via its phosphorylation. Phosphorylates PPP2R5B thereby stimulating the assembly of PP2A phosphatase with the PPP2R5B-AKT1 complex leading to dephosphorylation of AKT1. Phosphorylates: PTPN1, SRSF1 and SRSF3. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.