TRI18 Colorimetric Cell-Based ELISA
The TRI18 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor TRI18 protein expression profile in cells. The kit can be used for measuring the relative amounts of TRI18 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on TRI18.
Product Specifications
Synonyms
E3 ubiquitin-protein ligase Midline-1;2.3.2.27; Midin; Putative transcription factor XPRF; RING finger protein 59; RING finger protein Midline-1; RING-type E3 ubiquitin transferase Midline-1 ; Tripartite motif-containing protein 18; MID1; FXY, RNF59, TRIM18, XPRF
Gene Name
MID1
UniProt
O15344
Reactivity
Human, Mouse, Rat
Tissue Specificity
In the fetus, highest expression found in kidney, followed by brain and lung. Expressed at low levels in fetal liver. In the adult, most abundant in heart, placenta and brain.
Applications
ELISA
Detection Range
> 5000 cells/well
Function
Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination.
Molecular Weight
75251 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
E3 ubiquitin-protein ligase Midline-1
Subcellular Location
Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle. Microtubule-associated. It is associated with microtubules throughout the cell cycle, co-localizing with cytoplasmic fibers in interphase and with the mitotic spindle and midbodies during mitosis and cytokinesis.
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