TRI18 Colorimetric Cell-Based ELISA

The TRI18 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor TRI18 protein expression profile in cells. The kit can be used for measuring the relative amounts of TRI18 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on TRI18.

Product Specifications

Synonyms

E3 ubiquitin-protein ligase Midline-1;2.3.2.27; Midin; Putative transcription factor XPRF; RING finger protein 59; RING finger protein Midline-1; RING-type E3 ubiquitin transferase Midline-1 ; Tripartite motif-containing protein 18; MID1; FXY, RNF59, TRIM18, XPRF

Gene Name

MID1

UniProt

O15344

Reactivity

Human, Mouse, Rat

Tissue Specificity

In the fetus, highest expression found in kidney, followed by brain and lung. Expressed at low levels in fetal liver. In the adult, most abundant in heart, placenta and brain.

Applications

ELISA

Detection Range

> 5000 cells/well

Function

Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination.

Molecular Weight

75251 MW

Shipping Conditions

Available

Storage Conditions

Store at 4°C for up to 6 months.

Other Gene Names

E3 ubiquitin-protein ligase Midline-1

Subcellular Location

Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle. Microtubule-associated. It is associated with microtubules throughout the cell cycle, co-localizing with cytoplasmic fibers in interphase and with the mitotic spindle and midbodies during mitosis and cytokinesis.