Vimentin Colorimetric Cell-Based ELISA Kit

The Vimentin Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Vimentin protein expression profile in cells. The kit can be used for measuring the relative amounts of Vimentin in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Vimentin.

Product Specifications

Synonyms

Vimentin; VIM

Gene Name

VIM

UniProt

P08670

Reactivity

Human, Mouse, Rat

Tissue Specificity

Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines.

Applications

ELISA

Detection Range

> 5000 cells/well

Function

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.